THE EFFECT OF MORINGA LAM LEAF EXTRACT ON PHAGOCYTOSIS ACTIVITY OF MACROPHAGES, ROS, NO, IFN-γ, AND IL-10 ON PBMC IN ADULT PULMONARY TUBERCULOSIS PATIENTS INDUCED BY ESAT-6 IN-VITRO

Introduction: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB). MTB can inhibit phagolysosomal fusion, so it’s difficult to eliminate, and the BCG vaccine didn’t contain ESAT-6/CFP-10, which therapy immunization couldn’t be optimal in eradicating TB. An immunomodulator needed improve immune system, especially from natural ingredients. A Moringa oleifera plant known have immunomodulatory properties because of its nutrients, flavonoids, polyphenols, saponins,alkaloids, steroids.
 Material Methods: This study aims determine effect M. leaf extract (MLE) on phagocytic activity macrophages, ros, no, Ifn-γ, IL-10 induced ESAT-6 Peripheral Blood Mononuclear Culture (PBMC) pulmonary TB patients. The research was carried out PBMC blood six patients with a post-test-only control group design. samples were divided into three groups: negative group, positive (induced ESAT-6), intervention groups MLE at doses 1 μg/mL, 2.5 10 25 μg/mL).
 Results: dose μg/mL significantly increased macrophages’ activity, ros/no, decreased levels (p < 0.05) as compared whereas ≥ it macrophages levels.
 Discussion: These indicate that low had immunostimulant but higher showed immunosuppressive anti-inflammatory activity.